Non-enzymatic amperometric detection of hydrogen peroxide in human blood serum samples using a modified silver nanowire electrode

In this paper, we report a highly sensitive amperometric H₂O₂ sensor based on silver nanowires (AgNWs) modified screen printed carbon electrode. The AgNWs were synthesized using polyol method. The synthesized AgNWs were characterized by scanning electron microscopy, UV-vis spectroscopy and X-ray diffraction techniques. The average diameter and length of the synthesized AgNWs were found as 86 ± 5 and 385 nm, respectively. Under optimum conditions, the AgNWs modified electrode shows a stable amperometric response for H₂O₂ and was linear over the concentrations ranging from 0.3 to 704.8 μM. The non-enzymatic sensor showed a high sensitivity of 662.6 μA mM^-1 cm^-2 with a detection limit of 29 nM. The response time of the sensor was found as 2 s. Furthermore, the AgNWs modified electrode exhibited a good recovery of H₂O₂ (94.3%) in the human blood serum samples.