Micro Q-PCR chip on a miniaturized detection system for DNA detection and quantification

Jung Hao Wang; Liang Ju Chien; Tsung Min Hsieh; Ching Hsing Luo; Ping Hei Chen; Pei Jer Chen; Da Sheng Lee; Gwo Bin Lee

Micro Q-PCR chip on a miniaturized detection system for DNA detection and quantification

Jung Hao Wang
, Liang Ju Chien
, Tsung Min Hsieh
, Ching Hsing Luo
, Ping Hei Chen
, Pei Jer Chen
, Da Sheng Lee
, Gwo Bin Lee

Department of Energy and Refrigerating Air-Conditioning Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

In this study, a miniature quantitative real-time polymerase chain reaction (Q-PCR) detection system was demonstrated to allow for the detection of infectious diseases. A new flow-through PCR chips with three serpentine-shape (S-shape) pneumatic micro-pumps were designed to perform real-time PCR in an automatic format. The new design of array-type microheaters was adopted to improve the thermal uniformity. The fluores-cence-based detection method was employed. With the integration of the fluorescence detection system, quantitative detection of hepatitis B virus (HBV) can be achieved.

Original language	English
Pages	1054-1056
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 12 Oct 2008 → 16 Oct 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	12/10/08 → 16/10/08

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

MEMS
Microfluidics
Micropumps
Q-PCR

Cite this

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title = "Micro Q-PCR chip on a miniaturized detection system for DNA detection and quantification",

abstract = "In this study, a miniature quantitative real-time polymerase chain reaction (Q-PCR) detection system was demonstrated to allow for the detection of infectious diseases. A new flow-through PCR chips with three serpentine-shape (S-shape) pneumatic micro-pumps were designed to perform real-time PCR in an automatic format. The new design of array-type microheaters was adopted to improve the thermal uniformity. The fluores-cence-based detection method was employed. With the integration of the fluorescence detection system, quantitative detection of hepatitis B virus (HBV) can be achieved.",

keywords = "MEMS, Microfluidics, Micropumps, Q-PCR",

author = "Wang, \{Jung Hao\} and Chien, \{Liang Ju\} and Hsieh, \{Tsung Min\} and Luo, \{Ching Hsing\} and Chen, \{Ping Hei\} and Chen, \{Pei Jer\} and Lee, \{Da Sheng\} and Lee, \{Gwo Bin\}",

year = "2008",

language = "???core.languages.en\_GB???",

pages = "1054--1056",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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Micro Q-PCR chip on a miniaturized detection system for DNA detection and quantification. / Wang, Jung Hao; Chien, Liang Ju; Hsieh, Tsung Min et al.
2008. 1054-1056 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Micro Q-PCR chip on a miniaturized detection system for DNA detection and quantification

AU - Wang, Jung Hao

AU - Chien, Liang Ju

AU - Hsieh, Tsung Min

AU - Luo, Ching Hsing

AU - Chen, Ping Hei

AU - Chen, Pei Jer

AU - Lee, Da Sheng

AU - Lee, Gwo Bin

PY - 2008

Y1 - 2008

N2 - In this study, a miniature quantitative real-time polymerase chain reaction (Q-PCR) detection system was demonstrated to allow for the detection of infectious diseases. A new flow-through PCR chips with three serpentine-shape (S-shape) pneumatic micro-pumps were designed to perform real-time PCR in an automatic format. The new design of array-type microheaters was adopted to improve the thermal uniformity. The fluores-cence-based detection method was employed. With the integration of the fluorescence detection system, quantitative detection of hepatitis B virus (HBV) can be achieved.

AB - In this study, a miniature quantitative real-time polymerase chain reaction (Q-PCR) detection system was demonstrated to allow for the detection of infectious diseases. A new flow-through PCR chips with three serpentine-shape (S-shape) pneumatic micro-pumps were designed to perform real-time PCR in an automatic format. The new design of array-type microheaters was adopted to improve the thermal uniformity. The fluores-cence-based detection method was employed. With the integration of the fluorescence detection system, quantitative detection of hepatitis B virus (HBV) can be achieved.

KW - MEMS

KW - Microfluidics

KW - Micropumps

KW - Q-PCR

UR - https://www.scopus.com/pages/publications/84984548395

M3 - ???researchoutput.researchoutputtypes.contributiontoconference.paper???

AN - SCOPUS:84984548395

SP - 1054

EP - 1056

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Micro Q-PCR chip on a miniaturized detection system for DNA detection and quantification

Abstract

Conference

UN SDGs

Keywords

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