Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on reduced graphene oxide and silver nanoparticles nanocomposite modified electrode

Selvakumar Palanisamy; Chelladurai Karuppiah; Shen Ming Chen

doi:10.1016/j.colsurfb.2013.10.006

Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on reduced graphene oxide and silver nanoparticles nanocomposite modified electrode

Selvakumar Palanisamy
, Chelladurai Karuppiah
, Shen Ming Chen

Department of Chemical Engineering and Biotechnology

Research output: Contribution to journal › Article › peer-review

149 Scopus citations

Abstract

The direct electrochemistry of glucose oxidase (GOx) was successfully realized on electrochemically reduced graphene oxide and silver nanoparticles (RGO/Ag) nanocomposite modified electrode. The fabricated nanocomposite was characterized by field emission scanning electron microscope and energy dispersive spectroscopy. The GOx immobilized nanocomposite modified electrode showed a pair of well-defined redox peaks with a formal potential (E°) of -0.422V, indicating that the bioactivity of GOx was retained. The heterogeneous electron transfer rate constant (K_s) of GOx at the nanocomposite was calculated to be 5.27s^-1, revealing a fast direct electron transfer of GOx. The GOx immobilized RGO/Ag nanocomposite electrode exhibited a good electrocatalytic activity toward glucose over a linear concentration range from 0.5 to 12.5mM with a detection limit of 0.16mM. Besides, the fabricated biosensor showed an acceptable sensitivity and selectivity for glucose.

Original language	English
Pages (from-to)	164-169
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	114
DOIs	https://doi.org/10.1016/j.colsurfb.2013.10.006
State	Published - 1 Feb 2014

Bibliographical note

Funding Information:
This project was supported by the National Science Council and the Ministry of Education of Taiwan (Republic of China) .

Keywords

Direct electron transfer
Glucose biosensor
Glucose oxidase
Reduced graphene oxide
Silver nanoparticles

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10.1016/j.colsurfb.2013.10.006

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title = "Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on reduced graphene oxide and silver nanoparticles nanocomposite modified electrode",

abstract = "The direct electrochemistry of glucose oxidase (GOx) was successfully realized on electrochemically reduced graphene oxide and silver nanoparticles (RGO/Ag) nanocomposite modified electrode. The fabricated nanocomposite was characterized by field emission scanning electron microscope and energy dispersive spectroscopy. The GOx immobilized nanocomposite modified electrode showed a pair of well-defined redox peaks with a formal potential (E°) of -0.422V, indicating that the bioactivity of GOx was retained. The heterogeneous electron transfer rate constant (Ks) of GOx at the nanocomposite was calculated to be 5.27s-1, revealing a fast direct electron transfer of GOx. The GOx immobilized RGO/Ag nanocomposite electrode exhibited a good electrocatalytic activity toward glucose over a linear concentration range from 0.5 to 12.5mM with a detection limit of 0.16mM. Besides, the fabricated biosensor showed an acceptable sensitivity and selectivity for glucose.",

keywords = "Direct electron transfer, Glucose biosensor, Glucose oxidase, Reduced graphene oxide, Silver nanoparticles",

author = "Selvakumar Palanisamy and Chelladurai Karuppiah and Chen, \{Shen Ming\}",

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Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on reduced graphene oxide and silver nanoparticles nanocomposite modified electrode. / Palanisamy, Selvakumar; Karuppiah, Chelladurai; Chen, Shen Ming.
In: Colloids and Surfaces B: Biointerfaces, Vol. 114, 01.02.2014, p. 164-169.

Research output: Contribution to journal › Article › peer-review

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T1 - Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on reduced graphene oxide and silver nanoparticles nanocomposite modified electrode

AU - Palanisamy, Selvakumar

AU - Karuppiah, Chelladurai

AU - Chen, Shen Ming

N1 - Funding Information: This project was supported by the National Science Council and the Ministry of Education of Taiwan (Republic of China) .

PY - 2014/2/1

Y1 - 2014/2/1

N2 - The direct electrochemistry of glucose oxidase (GOx) was successfully realized on electrochemically reduced graphene oxide and silver nanoparticles (RGO/Ag) nanocomposite modified electrode. The fabricated nanocomposite was characterized by field emission scanning electron microscope and energy dispersive spectroscopy. The GOx immobilized nanocomposite modified electrode showed a pair of well-defined redox peaks with a formal potential (E°) of -0.422V, indicating that the bioactivity of GOx was retained. The heterogeneous electron transfer rate constant (Ks) of GOx at the nanocomposite was calculated to be 5.27s-1, revealing a fast direct electron transfer of GOx. The GOx immobilized RGO/Ag nanocomposite electrode exhibited a good electrocatalytic activity toward glucose over a linear concentration range from 0.5 to 12.5mM with a detection limit of 0.16mM. Besides, the fabricated biosensor showed an acceptable sensitivity and selectivity for glucose.

AB - The direct electrochemistry of glucose oxidase (GOx) was successfully realized on electrochemically reduced graphene oxide and silver nanoparticles (RGO/Ag) nanocomposite modified electrode. The fabricated nanocomposite was characterized by field emission scanning electron microscope and energy dispersive spectroscopy. The GOx immobilized nanocomposite modified electrode showed a pair of well-defined redox peaks with a formal potential (E°) of -0.422V, indicating that the bioactivity of GOx was retained. The heterogeneous electron transfer rate constant (Ks) of GOx at the nanocomposite was calculated to be 5.27s-1, revealing a fast direct electron transfer of GOx. The GOx immobilized RGO/Ag nanocomposite electrode exhibited a good electrocatalytic activity toward glucose over a linear concentration range from 0.5 to 12.5mM with a detection limit of 0.16mM. Besides, the fabricated biosensor showed an acceptable sensitivity and selectivity for glucose.

KW - Direct electron transfer

KW - Glucose biosensor

KW - Glucose oxidase

KW - Reduced graphene oxide

KW - Silver nanoparticles

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ER -

Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on reduced graphene oxide and silver nanoparticles nanocomposite modified electrode

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