Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica

Shih Yu Liang; Yun Hsien Chan; Kan Tai Hsia; Jing Lun Lee; Ming Chu Kuo; Kuo Yuan Hwa; Chi Wen Chan; Ting Yi Chiang; Jung Sheng Chen; Fang Tzy Wu; Dar Der Ji

doi:10.1128/JCM.00105-09

Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica

Shih Yu Liang
, Yun Hsien Chan
, Kan Tai Hsia
, Jing Lun Lee
, Ming Chu Kuo
, Kuo Yuan Hwa
, Chi Wen Chan
, Ting Yi Chiang
, Jung Sheng Chen
, Fang Tzy Wu
, Dar Der Ji

Department of Molecular Science and Engineering

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.

Original language	English
Pages (from-to)	1892-1895
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	47
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00105-09
State	Published - Jun 2009

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title = "Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica",

abstract = "A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.",

author = "Liang, \{Shih Yu\} and Chan, \{Yun Hsien\} and Hsia, \{Kan Tai\} and Lee, \{Jing Lun\} and Kuo, \{Ming Chu\} and Hwa, \{Kuo Yuan\} and Chan, \{Chi Wen\} and Chiang, \{Ting Yi\} and Chen, \{Jung Sheng\} and Wu, \{Fang Tzy\} and Ji, \{Dar Der\}",

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T1 - Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica

AU - Liang, Shih Yu

AU - Chan, Yun Hsien

AU - Hsia, Kan Tai

AU - Lee, Jing Lun

AU - Kuo, Ming Chu

AU - Hwa, Kuo Yuan

AU - Chan, Chi Wen

AU - Chiang, Ting Yi

AU - Chen, Jung Sheng

AU - Wu, Fang Tzy

AU - Ji, Dar Der

PY - 2009/6

Y1 - 2009/6

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AB - A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.

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ER -

Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica

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