Development of a multiple biosensor and its application of biofuel cell

The gold nanoparticles (GNP) and functionalized carbon nanotube (f-CNT) film (GNP-f-CNT) was prepared on glassy carbon electrode (GCE) by multiple scan cyclic voltammetry. The glucose oxidase (GOx) was physical adsorbed on the GNP-f-CNT film surface. The electrochemical measurements and surface morphology of the as-prepared films were studied using field emission scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD). The proposed film were demonstrated for the determination of dissolved oxygen using linear sweep voltammetry (LSV) and rotating disk electrode voltammetry (RDE). The proposed electrodes for the construction of biofuel cell (BFC) was achieved up to 22.6 μW cm^-2, and worked well as a biosensor for both glucose and dissolved oxygen with high sensitivity of 281.86 μA mM^-1 cm^-2 and 205.73 μA L mg^-1 cm^-2, respectively. The proposed protocol for synthesizing the GOx/GNP-f-CNT bionanocomposite is simple, convenient and fast in operation, which is expected to find wide biosensing and bioelectronic applications.